Array-based sensing of proteins using conjugated polymers

作者:Miranda Oscar R; You Chang Cheng; Phillips Ronnie; Kim Ik Bum; Ghosh Partha S; Bunz Uwe H F*; Rotello Vincent M
来源:Journal of the American Chemical Society, 2007, 129(32): 9856-+.
DOI:10.1021/ja0737927

摘要

A sensor array comprising six functionalized poly(p-phenyleneethynylene) polymers was constructed and used to detect and identify protein analytes. The presence of proteins alters the fluorescence properties of the polymers, generating a distinct fluorescence response pattern for a given protein. Linear discriminant analyses accurately differentiate the patterns of 17 proteins at nano- to micromolar concentrations. An identification accuracy of 97% was obtained on the basis of the tests with 68 unknown protein samples from the training set.

  • 出版日期2007-8-15