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摘要
Background Interstitial cells of Cajal associated with the submuscular plexus (ICC-SMP) generate omni-present slow-wave activity in the colon and are associated with prominent motor patterns. Our aim was to investigate colon motor dysfunction in W/W-v mice in which the ICC are reportedly reduced. Methods Whole organ colon motility was studied using spatio-temporal mapping; immunohistochemical staining was carried out for c-Kit and Ano1; calcium imaging was applied to ICC-SMP. Key Results Discrepancies between Ano1 and c-Kit staining were found in both wild-type and W/W-v colon. ICC-SMP were reduced to similar to 50% in the W/W-v mouse colon according to c-Kit immunohistochemistry, but Ano1 staining indicated a normal network of ICC-SMP. The latter was consistent with rhythmic calcium transients occurring at the submucosal border of the colon in W/W-v mice, similar to the rhythmic transients in wild-type ICC-SMP. Furthermore, the motor pattern associated with ICC-SMP pacemaking, the so-called 'ripples' were normal in the W/W-v colon. Conclusions & Inferences c-Kit is not a reliable marker for quantifying ICC-SMP in the mouse colon. Ano1 staining revealed a normal network of ICC-SMP consistent with the presence of a normal 'ripples' motor pattern. We detected a class of Ano1 positive c-Kit negative cells that do not depend on Kit expression for maintenance, a feature shared with ICC progenitors.
- 出版日期2014-9
- 单位武汉大学
