Monitoring enzymatic ATP hydrolysis by EPR spectroscopy

作者:Hacker Stephan M*; Hintze Christian; Marx Andreas; Drescher Malte
来源:Chemical communications, 2014, 50(55): 7262-7264.
DOI:10.1039/c4cc02422b

摘要

An adenosine triphosphate (ATP) analogue modified with two nitroxide radicals is developed and employed to study its enzymatic hydrolysis by electron paramagnetic resonance spectroscopy. For this application, we demonstrate that EPR holds the potential to complement fluorogenic substrate analogues in monitoring enzymatic activity.

  • 出版日期2014