BSH-CP based 3D solid-state NMR experiments for protein resonance assignment

作者：Shi Chaowei; Fasshuber Hannes K; Chevelkov Veniamin; Xiang Shengqi; Habenstein Birgit; Vasa Suresh Kumar; Becker Stefan; Lange Adam^*

来源：Journal of Biomolecular NMR, 2014, 59(1): 15-22.

DOI：10.1007/s10858-014-9820-8

• Solid-state NMR
• Magic-angle spinning
• BSH-CP
• Protein resonance assignment
• Ubiquitin

We have recently presented band-selective homonuclear cross-polarization (BSH-CP) as an efficient method for CO-CA transfer in deuterated as well as protonated solid proteins. Here we show how the BSH-CP CO-CA transfer block can be incorporated in a set of three-dimensional (3D) solid-state NMR (ssNMR) pulse schemes tailored for resonance assignment of proteins at high static magnetic fields and moderate magic-angle spinning rates. Due to the achieved excellent transfer efficiency of 33 % for BSH-CP, a complete set of 3D spectra needed for unambiguous resonance assignment could be rapidly recorded within 1 week for the model protein ubiquitin. Thus we expect that BSH-CP could replace the typically used CO-CA transfer schemes in well-established 3D ssNMR approaches for resonance assignment of solid biomolecules.

• 出版日期2014-5
• 单位中国科学技术大学