Assessing loss of imprint methylation in sperm from subfertile men using novel methylation polymerase chain reaction Luminex analysis

作者：Sato Akiko; Hiura Hitoshi; Okae Hiroaki; Miyauchi Naoko; Abe Yukiko; Utsunomiya Takafumi; Yaegashi Nobuo; Arima Takahiro^*

来源：Fertility and Sterility, 2011, 95(1): 129-134.

DOI：10.1016/j.fertnstert.2010.06.076

• Bisulfite PCR-Luminex methylation analysis
• BPL
• DNA methylation
• genomic imprinting
• oligozoospermia
• sperm

Objective: To assess the clinical value of bisulfite polymerase chain reaction Luminex (BPL), an automated, high-throughput procedure for the detection of alterations in DNA methylation.
Design: Experimental prospective study.
Setting: University research laboratory and private in vitro fertilization (IVF) clinic.
Patient(s): A total of 337 men, 61 with severe oligozoospermia, 67 with moderate oligozoospermia, and 209 with microscopically normozoospermia.
Intervention(s): The ejaculated sperm samples after the routine semen analysis with patients' consent.
Main Outcome Measure(s): Examination of the methylation patterns of eight imprinted loci in sperm DNA, and confirmation with combined bisulfite PCR restriction analysis (COBRA).
Result(s): A total of 47 cases (13.9%) showed abnormal methylation at one or more imprinted loci (18 paternal, 18 maternal, and 11 cases with alterations of both maternal and paternal imprints).
Conclusion(s): The relative ease of the BPL method provides a practical method within a clinical setting to reduce the likelihood of abnormal samples being used in assisted reproduction treatments. (Fertil Steril(R) 2011;95:129-34.

• 出版日期2011-1