A novel signal amplification strategy of an electrochemical aptasensor for kanamycin, based on thionine functionalized graphene and hierarchical nanoporous PtCu

作者：Qin Xiaoli; Yin Yan; Yu Huijing; Guo Wenjuan^*; Pei Meishan

来源：Biosensors and Bioelectronics, 2016, 77: 752-758.

DOI：10.1016/j.bios.2015.10.050

• Electrochemical aptasensor
• Thionine
• Platinum
• Kanamycin

An ultrasensitive electrochemical aptasensor for the quantitative detection of kanamycin antibiotic was fabricated based on a novel signal amplification strategy. This aptasensor was developed using thionine functionalized graphene (GR-TH) and hierarchical nanoporous (HNP) PtCu alloy as biosensing substrates for the first time. HNP-PtCu alloy with controllable bimodal ligament/pore distributions was successfully prepared by two-step dealloying of a well-designed PtCuAl precursor alloy combined with an annealing operation. GR-TH composite was synthesized by one-step reduction of graphene oxide (GO) in TH solution. Greatly amplified sensitivity was achieved by using GR-TH/FINP-PtCu composite owing to its large specific surface and good electron-transfer ability. Under the optimized conditions, the proposed aptasensor exhibited a high sensitivity and a wider linearity to kanamycin in the range 5 x 10(-7)-5 x 10(-2) mu g mL(-1) with a low detection limit of 0.42 pg mL(-1). This aptasensor also displayed a satisfying electrochemical performance with good stability, selectivity and reproducibility. The as-prepared aptasensor was successfully used for the determination of kanamycin in animal derived food.

• 出版日期2016-3-15
• 单位济南大学