FGF-2 Angiogenesis in Bone Regeneration Within Critical-Sized Bone Defects in Rat Calvaria

作者：Kigami Risa; Sato Shuichi^*; Tsuchiya Noriko; Yoshimakai Tomohiro; Arai Yoshinori; Ito Koichi

来源：Implant Dentistry, 2013, 22(4): 422-427.

DOI：10.1097/ID.0b013e31829d19f0

• blood vessels
• fibroblast growth factor
• micro-CT

Objective: This study examined the effect of basic fibroblast growth factor (FGF)-2 with an absorbable collagen sponge on angiogenesis and bone regeneration in rat calvarial critical-sized bone defects using microcomputed tomography.Methods: Forty 7-week-old male Fischer rats were used. The symmetrical critical-sized calvarial bone defects (5 mm diameter) were created. An absorbable collagen sponge with or without FGF-2 (0.1% or 0.3%) was implanted into each bone defect.Results: Blood vessel and bone volumes were significantly higher in the 0.3% FGF-2 group compared with the control and 0.1% FGF-2 groups on day 28. Significantly more osteoblast- and osteoclast-like cells were seen in the 0.3% FGF-2 group.Conclusions: Thus, FGF-2 increased blood vessel and bone formation in rat calvarial critical-sized bone defects.

• 出版日期2013-8