A Reversible Association between Smc Coiled Coils Is Regulated by Lysine Acetylation and Is Required for Cohesin Association with the DNA

作者：Kulemzina Irina; Ang Keven; Zhao Xiaodan; Teh Jun Thing; Verma Vikash; Suranthran Sasikala; Chavda Alap P; Huber Roland G; Eisenhaber Birgit; Eisenhaber Frank; Yan Jie; Ivanov Dmitri^*

来源：Molecular Cell, 2016, 63(6): 1044-1054.

DOI：10.1016/j.molcel.2016.08.008

Cohesin is a ring-shaped protein complex that is capable of embracing DNA. Most of the ring circumference is comprised of the anti-parallel intramolecular coiled coils of the Smc1 and Smc3 proteins, which connect globular head and hinge domains. Smc coiled coil arms contain multiple acetylated and ubiquitylated lysines. To investigate the role of these modifications, we substituted lysines for arginines to mimic the unmodified state and uncovered genetic interaction between the Smc arms. Using scanning force microscopy, we show that wild-type Smc arms associate with each other when the complex is not on DNA. Deacetylation of the Smc1/Smc3 dimers promotes arms' dissociation. Smc arginine mutants display loose packing of the Smc arms and, although they dimerize at the hinges, fail to connect the heads and associate with the DNA. Our findings highlight the importance of a "collapsed ring,'' or "rod,'' conformation of cohesin for its loading on the chromosomes.

• 出版日期2016-9-15
• 单位南阳理工学院