Absorption Kinetics of the Main Conjugated Linoleic Acid Isomers in Commercial-Rich Oil after Oral Administration in Rats

作者：Rodriguez Alcala Luis M; Ares Irma; Fontecha Javier; Martinez Larranaga Maria Rosa; Anadon Arturo; Martinez Maria Aranzazu^*

来源：Journal of Agricultural and Food Chemistry, 2017, 65(35): 7680-7686.

DOI：10.1021/acs.jafc.7b02143

• CLA isomers
• RA
• C18:2 trans 10
• cis 12
• oral absorption
• plasma disposition
• rats

This study aimed to assess the oral absorption and plasma kinetics of two main isomers contained in commercial conjugated linoleic acid (CLA)-rich oil (Tonalin TG-80), rumenic acid (RA), and C18:2 trans-10, cis-12. The isomer plasma disposition after the single oral dose of 3000 mg of Tonalin TG-80/kg, containing 1200 mg/kg of each isomer, was studied in rats. The isomer plasma concentrations were determined by gas chromatography with flame ionization detection. The plasma kinetics showed rapid oral absorption of RA and C18:2 trans-10, cis-12 (t(1/2a) 0.34 +/- 0.09 and 0.53 +/- 0.01 h) and slow elimination (t(1/2 beta) 25.68 +/- 3.29 and 18.12 +/- 1.71 h); the maximal isomer plasma concentrations (C-max) of 8.48 +/- 0.98 and 7.67 +/- 0.80 mu g mL(-1), respectively, were estimated at 2.08 +/- 0.14 and 2.26 +/- 0.11 h. Our results from a preclinical kinetic study in rats help to design future studies in humans for evaluating the CLA isomer dose-response.

• 出版日期2017-9-6