ornyl acetate extracted from Sharen(Fructus Amomi) inhibits proliferation， invasion and induces apoptosis by suppressing phosphatidylinositol-3-kinase/protein kinase B signaling in colorectal cancer

作者：LI Xiaohua; DUAN Zhihang; YUE Jianjun; ZHANG Yongyu; LI Yihang; LIU Shifang; NIE Qu; YANG Depo^*; ZHANG Lixia^*

来源：Journal of Traditional Chinese Medicine, 2023, 1-11.

DOI：10.19852/j.cnki.jtcm.20231018.002

• bornyl acetate
• colorectal neoplasms
• phosphatidylinositol 3-kinase
• protein kinases B
• signal transduction

OBJECTIVE: To investigate the antitumor effects of bornyl acetate (BA) isolated from Sharen (Fructus Amomi) in colorectal cancer (CRC) and the underlying mechanisms. METHODS: SW480 and HT29 cells were treated with increasing doses of BA in order to determine its antitumor effects in vitro. Cell viability， colony formation， cell cycle， and apoptosis as well as migration and invasion were assessed using various assays. In addition， the in vivo antitumor effects of BA were assessed using a xenograft mouse model. We then assessed the mechanism of action of BA by conducting pathway activator-mediated rescue experiments and assessed the protein levels by Western blot analysis. RESULTS: BA showed anti-CRC tumor activities in vitro by suppressing cell proliferation and colony formation， inducing apoptosis， blocking cell cycle， and inhibiting migration and invasion. These effects were mediated via suppression of the PI3K/AKT pathway. In the tumor xenograft experiment， BA was found to repress tumor growth in vivo with low toxicity. CONCLUSIONS: The results demonstrated that BA exerts antitumor effects by suppressing the phosphatidylinositol-3-kinase/protein kinase B pathway， with low toxicity. Thus， BA might be a potential novel therapeutic agent for CRC. ? 2022 JTCM.

• 出版日期2023-10-20
• 单位中山大学; 中国医学科学院北京协和医院; 云南大学