Proteolytic Activity at Quantum Dot-Conjugates: Kinetic Analysis Reveals Enhanced Enzyme Activity and Localized Interfacial %26quot;Hopping%26quot;

作者：Algar W Russ; Malonoski Anthony; Deschamps Jeffrey R; Banco Canosa Juan B; Susumu Kimihiro; Stewart Michael H; Johnson Brandy J; Dawson Philip E; Medintz Igor L^*

来源：Nano Letters, 2012, 12(7): 3793-3802.

DOI：10.1021/nl301727k

• Quantum dot
• nanoparticle
• peptide
• proteolysis
• kinetics
• FRET

Recent studies show that polyvalent, ligand-modified nanoparticles provide significantly enhanced binding characteristics compared to isolated ligands. Here, we assess the ability of substrate-modified nanoparticles to provide enhanced enzymatic activity. Energy transfer assays allowed quantitative, real-time measurement of proteolytic digestion at polyvalent quantum dot-peptide conjugates. Enzymatic progress curves were analyzed using an integrated Michaelis Menten (MM) formalism, revealing mechanistic details, including deviations from classic MM-behavior. A %26quot;hopping%26quot; mode of proteolysis at the nanoparticle was identified, confirming enhanced activity.

• 出版日期2012-7