Antimicrobial Activity of the Scolopendrasin V Peptide Identified from the Centipede Scolopendra subspinipes mutilans

作者：Lee Joon Ha; Kim In Woo; Kim Mi Ae; Ahn Mi Young; Yun Eun Young; Hwang Jae Sam

来源：Journal of Microbiology and Biotechnology, 2017, 27(1): 43-48.

DOI：10.4014/jmb.1609.09057

• Antimicrobial peptide
• membrane damage
• bacterial membrane
• scolopendrasin V
• Scolopendra subspinipes mutilans

In a previous study, we analyzed the transcriptome of Scolopendra subspinipes mutilans using next-generation sequencing technology and identified several antimicrobial peptide candidates. One of the peptides, scolopendrasin V, was selected based on the physicochemical properties of antimicrobial peptides using a bioinformatics strategy. In this study, we assessed the antimicrobial activities of scolopendrasin V using the radial diffusion assay and colony count assay. We also investigated the mode of action of scolopendrasin V using flow cytometry. We found that scolopendrasin V's mechanism of action involved binding to the surface of microorganisms via a specific interaction with lipopolysaccharides, lipoteichoic acid, and peptidoglycans, which are components of the bacterial membrane. These results provide a basis for developing peptide antibiotics.

• 出版日期2017-1