Albumin resuscitation protects against traumatic/hemorrhagic shock-induced lung apoptosis in rats

作者：Zhang, Yun; Liang, Zhong-yan; Zhang, Shao-yang; Huang, Fang-fang; Wu, Wei; Gao, Yuan; Chen, Zuo-bing^*

来源：Journal of Zhejiang University-Science B(Biomedicine & Biotechnology), 2008, 9(11): 871-878.

DOI：10.1631/jzus.B0820130

• Traumatic/hemorrhagic shock (T/HS)
• Mitogen-activated protein kinase (MAPK)
• Nuclear factor kappa B (NF-kappa B)
• Albumin
• Apoptosis

Objective: To determine the effects of albumin administration on lung injury and apoptosis in traumatic/hemorrhagic shock (T/HS) rats. Methods: Studies were performed on an in vivo model of spontaneously breathing rats with induced T/HS; the rats were subjected to femur fracture, ischemia for 30 min, and reperfusion for 20 min with Ringer's lactate solution (RS) or 5% (w/v) albumin (ALB), and the left lower lobes of the lungs were resected. Results: Albumin administered during reperfusion markedly attenuated injury of the lung and decreased the concentration of lactic acid and the number of in situ TdT-mediated dUTP nick-end labelling (TUNEL)-positive cells. Moreover, immunohistochemistry performed 24 h after reperfusion revealed increases in the level of nuclear factor kappa B (NF-kappa B), and phosphorylated p38 mitogen-activated protein kinase (MAPK) in the albumin-untreated group was down-regulated by albumin treatment when compared with the sham rats. Conclusion: Resuscitation with albumin attenuates tissue injury and inhibits T/HS-induced apoptosis in the lung via the p38 MAPK signal transduction pathway that functions to stimulate the activation of NF-kappa B.

• 出版日期2008-11
• 单位浙江大学