Aginoside saponin, a potent antifungal compound, and secondary metabolite analyses from Allium nigrum L.

作者：Mostafa Abdelrahman; Sudisha Jogaiah; El Sayed Magdi; Ito Shin ichi; Ikeda Tsuyoshi; Yamauchi Naoki; Shigyo Masayoshi^*

来源：Phytochemistry Letters, 2013, 6(2): 274-280.

DOI：10.1016/j.phytol.2013.03.001

• Allium nigrum
• Saponin
• Aginoside
• Antifungal activity
• Cysteine sulfoxides
• Polyphenols

The HPLC and spectral analyses of cysteine sulfoxides (CSOs), total polyphenols (TP), and total saponins revealed quantitative variations within the different organs of Allium nigrum L. A large accumulation of CSOs was detected in the bulb (0.367 mg/g fw), of TP in the leaf (116.05 mg CE/100 g fw), and of saponins in the root (19.38 mg/g dw). Phytochemical and chromatographical investigations of A. nigrum root extract led to the isolation of a spirostane-type glycoside or aginoside. The structure was elucidated by spectroscopic analysis (2D NMR, FABMS, HR-ESI-MS). The structure of the aginoside was identified as 25(R,S)-5 alpha-spirostan-2 alpha,3 beta,6 beta-trio1-3-O-beta-D-glucopyranosyl-(1 -%26gt; 2)-O-[beta-D-xylopyranosyl-(1 -%26gt; 3)]-O-beta-D-glucopyranosyl-(1 -%26gt; 4)-beta-D-galactopyranoside. The highest content of aginoside, 2.9 mg/g dw, was detected in the root. The in vitro and in vivo antifungal activity of aginoside was evaluated for the first time against phytopathogens. This compound showed significant (P %26lt; 0.05) antifungal activity depending on the concentration.

• 出版日期2013-5