Anti-inflammatory phenolics isolated from Juniperus rigida leaves and twigs in lipopolysaccharide-stimulated RAW264.7 macrophage cells

作者：Jeong Eun Ju; Seo Hanee; Yang Heejung; Kim Jinwoong; Sung Sang Hyun; Kim Young Choong^*

来源：Journal of Enzyme Inhibition and Medicinal Chemistry, 2012, 27(6): 875-879.

DOI：10.3109/14756366.2011.625025

• Juniperus rigida
• Cupressaceae
• nitric oxide
• inflammation
• RAW264.7

Inflammation is an essential host defense system particularly in response to infection and injury; however, excessive or undesirable inflammatory responses contribute to acute and chronic human diseases. A high-throughput screening effort searching for anti-inflammatory compounds from medicinal plants deduced that the methanolic extract of Juniperus rigida S. et L. (Cupressaceae) inhibited significantly nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Activity-guided fractionation and isolation yielded 13 phenolic compounds, including one new phenylpropanoid glycosides, 3,4-dimethoxycinnamyl 9-O-beta-D-glucopyranoside (1). Among the isolated compounds, phenylpropanoid glycosides with p-hydroxy group (2, 4) and massoniaside A (7), (+)-catechin (10), amentoflavone (11) effectively inhibited LPS-induced NO production in RAW264.7 cells.

• 出版日期2012-12