Application of open porous poly(D,L-lactide-co-glycolide) microspheres and the strategy of hydrophobic seeding in hepatic tissue cultivation

作者：Chou Ming Ju; Hsieh Chin Hsiung; Yeh Peng Lin; Chen Po Cheng; Wang Ching Hua; Huang Yi You^*

来源：Journal of Biomedical Materials Research Part A, 2013, 101(10): 2862-2869.

DOI：10.1002/jbm.a.34594

• hepatocyte cultivation
• porous PLGA microspheres
• 3D-coculture
• HUVEC
• BMMSC

In this article, porous poly(D,L-lactide-co-glycolide) (PLGA) microsphere scaffolds with a size of approximate to 400 m and pores of approximate to 20 m were prepared for constructing injectable three-dimensional hepatocyte spheroids. The porous sites of PLGA microspheres provided a spatial space for hepatocyte distribution. Hepatocytes spheroids were cocultured with human umbilical vein endothelial cell, bone marrow mesenchymal stem cell, or NIH/3T3 cells by combining the porous PLGA microspheres with the relatively hydrophobic culture strategy. The combination of open porous microspheres, hepatocytes, and nonparenchymal cells was demonstrated for application in functional hepatic tissue reconstruction. Hepatocellular-specific functions can sustained up to 2 weeks in the support of coculturing with nonparenchymal cells. The spheroidal hepatocyte coculture system had the advantages of an injectable delivery, higher cell seeding density, protection from exerted shear stress, better exchange of nutrients, oxygen and metabolites, and heterotypic cell-cell contact within and between microspheres.

• 出版日期2013-10