A novel "donor-two-acceptor" type fluorophore-based probe for fast detection and intracellular imaging of nitroreductase

作者：Li, Dandan; Xu, Yuqiong; Zhou, Nannan; Liu, Jianxu; Wang, Rui; Cheng, Tao; Tang, Yun; Zhu, Weiping; Xu, Yufang^*; Qian, Xuhong^*

来源：Dyes and Pigments, 2017, 136: 627-632.

DOI：10.1016/j.dyepig.2016.09.014

• FLUORESCENT-PROBE
• SELECTIVE DETECTION
• HYDROGEN-SULFIDE
• AQUEOUS-SOLUTION
• FAR-RED
• HYPOXIA
• CYANINE
• ESIPT
• CHEMODOSIMETER
• HYDRAZINE

By fusion of HBT and hemicyanine, we have designed a novel donor-two-acceptor type fluorophore (HHC) as a platform for the detection of different kinds of guests. HHC displays an emission at 580 nm in aqueous solution and two emission bands at 450 nm and 616 nm as a result of the donor-two-acceptor feature with the existance of CTAB. The fluorescence of HHC may be readily modulated by protection/deprotection of its electron-donating hydroxyl group and response different guests. As an example, we introduced p-nitrobenzyl group, which is sensitive to Nitroreductase (NTR), to the hydroxyl group of HHC and constructed a novel selective and sensitive fluorescence probe HHCP for fast detection and intracellular imaging of nitroreductase.

• 出版日期2017-1
• 单位生物反应器工程国家重点实验室; 华东理工大学