Detection and Quantification of Sulfonamide Antibiotic Residues in Milk Using Scanning Electrochemical Microscopy

作者：Conzuelo Felipe; Stratmann Lutz; Gruetzke Stefanie; Pingarron Jose M; Schuhmann Wolfgang^*

来源：Electroanalysis, 2014, 26(3): 481-487.

DOI：10.1002/elan.201300577

• Carbon surface modification
• Integrated immunosensor
• Milk
• Scanning electrochemical microscopy
• Sulfonamide antibiotics

The use of scanning electrochemical microscopy (SECM) for the qualitative and quantitative determination of sulfapyridine (SPY) in milk is described. A direct competitive immunoassay was performed involving an antibiotic horseradish peroxidase (HRP)-labeled analog and using selective capture antibodies immobilized on the surface of Protein G-modified glassy carbon plates. SECM detection was accomplished by means of the sample generator/tip collector (GC) mode involving the reduction of benzoquinone (BQ) generated upon the HRP-catalyzed oxidation of hydroquinone (HQ) at the modified substrate surface in the presence of H2O2. The detection limit for SPY in milk samples was as low as 0.13ngmL(-1).

• 出版日期2014-3