科研之友
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摘要
We have shown that the intracellular pH of a single HeLa cell expressing the enhanced green fluorescent protein ( EGFP) can be imaged using the fluorescence lifetime of EGFP, which can be interpreted in terms of the pH-dependent ionic equilibrium of the p-hydroxybenzylidene- imidazolidinone structure of the chromophore of EGFP.
- 出版日期2008
- 单位浙江大学
