Assignment of the orphan nuclear receptor Nurr1 by NMR

作者：Michiels Paul; Atkins Karen; Ludwig Christian; Whittaker Sara; van Dongen Maria; Guenther Ulrich

来源：Biomolecular NMR Assignments, 2010, 4(1): 101-105.

DOI：10.1007/s12104-010-9210-4

• Orphan nuclear receptor
• NMR
• Nurr1
• Parkinson's disease
• NR4A nuclear receptors

The orphan nuclear receptor Nurr1 has been implicated in a number of conditions including Parkinson's disease and Schizophrenia. As such, it is of interest to study its interactions with other proteins, possibly mediated by small molecules, considering possible use as a drug target. We produced (2)H, (15)N, (13)C labelled-Nurr1 to generate the backbone amide NH, carbonyl C', C(alpha) and C(beta) assignments. About 84.0% of residues could be assigned. Most of the 37 missing assignments fall in 3 regions of the protein. Two of these surround a putative ligand-binding region of Nurr1, suggesting that this region of the protein is flexible, despite the ligand-binding pocket being filled with hydrophobic side-chains from residues surrounding the ligand binding pocket.

• 出版日期2010-4