A Whole-Brain Atlas of Inputs to Serotonergic Neurons of the Dorsal and Median Raphe Nuclei

作者：Dorocic, Iskra Pollak; Furth, Daniel; Xuan, Yang; Johansson, Yvonne; Pozzi, Laura; Silberberg, Gilad; Carlen, Marie; Meletis, Konstantinos^*

来源：Neuron, 2014, 83(3): 663-678.

DOI：10.1016/j.neuron.2014.07.002

• VENTRAL TEGMENTAL AREA
• GENE-EXPRESSION
• PREFRONTAL CORTEX
• LATERAL HABENULA
• SUBSTANTIA-NIGRA
• MOUSE-BRAIN
• ASCENDING PROJECTIONS
• FUNCTIONAL TOPOGRAPHY
• EFFERENT CONNECTIONS
• GLOBUS-PALLIDUS

The serotonin system is proposed to regulate physiology and behavior and to underlie mood disorders; nevertheless, the circuitry controlling serotonergic neurons remains uncharacterized. We therefore generated a comprehensive whole-brain atlas defining the monosynaptic inputs onto forebrain-projecting serotonergic neurons of dorsal versus median raphe based on a genetically restricted transsynaptic retrograde tracing strategy. We identified discrete inputs onto serotonergic neurons from forebrain and brainstem neurons, with specific inputs from hypothalamus, cortex, basal ganglia, and midbrain, displaying a greater than anticipated complexity and diversity in cell-type-specific connectivity. We identified and functionally confirmed monosynaptic glutamatergic inputs from prefrontal cortex and lateral habenula onto serotonergic neurons as well as a direct GABAergic input from striatal projection neurons. In summary, our findings emphasize the role of hyperdirect inputs to serotonergic neurons. Cell-type-specific classification of connectivity patterns will allow for further functional analysis of the diverse but specific inputs that control serotonergic neurons during behavior.

• 出版日期2014-8-6