Differentiation of human umbilical cord-derived mesenchymal stem cells into hepatocytes in vitro

作者：Zheng, Guan; Liu, Yang; Jing, Qian; Zhang, Lei^*

来源：Bio-medical Materials and Engineering, 2015, 25(s1): S145-S157.

DOI：10.3233/BME-141249

• Human umbilical cord-derived mesenchymal stem cells (HUC-MSCs)
• hepatocytic differentiation
• hepatocytes

OBJECTIVE: The stem cell based therapy is a potential alternative to liver transplantation. The aim of the study is to investigate the hepatocytic differentiation ability of human umbilical cord derived mesenchymal stem cells (HUC-MSCs) in vitro.
METHODS: The HUC-MSCs were isolated from Wharton's jelly of human umbilical cord. The cells were identified by assessing the stem cell markers. The HUC-MSCs were characterized by multipotency of differentiation. We modified the hepatogenic differentiation protocol and examine the function of differentiated cell by Periodic acid-Schiff (PAS) staining and Low-Density Lipoprotein (LDL) uptake. The protein expressions of Total protein (TP), Albumin (ALB), Globulin (GLB), Urea (BUN) and a-fetoprotein (AFP) were also detected.
RESULTS: The cells of the hepatogenic differentiation group showed the function of hepatocyte. Protein expressions of TP, ALB, GLB BUN and AFP improve that the HUC-MSCs are able to different into the functional hepatogenic-like cell.
CONCLUSION: The above founding indicated that the HUC-MSCs are able to different into the functional hepatogenic-like cell by present method.

• 出版日期2015
• 单位昆明医科大学