Aqueous Two-Phase Extraction Combined with Chromatography: New Strategies for Preparative Separation and Purification of Capsaicin from Capsicum Oleoresin

作者：Zhao Pei pei; Lu Yan min; Tan Cong ping; Liang Yan; Cui Bo^*

来源：Applied Biochemistry and Biotechnology, 2015, 175(2): 1018-1034.

DOI：10.1007/s12010-014-1303-6

• Capsaicin
• Separation
• Aqueous two-phase extraction
• Purification
• Chromatography
• Reverse-phase resin

Capsaicin was preparatively separated and purified from capsicum oleoresin with a new method combined with aqueous two-phase extraction (ATPE) and chromatography. Screening experiments of ATPE systems containing salts and hydrophilic alcohols showed that potassium carbonate/ethanol system was the most suitable system for capsaicin recovery among the systems considered. Response surface methodology was used to determine an optimized aqueous two-phase system for the extraction of capsaicin from capsaicin oleoresin. In a 20 % (w/w) ethanol/22.3 % (w/w) potassium carbonate system, 85.4 % of the capsaicin was recovered in the top ethanol-rich phase while most oil and capsanthin ester were removed in the interphase. The capsaicinoid extract was then subjected to two chromatographic steps using D101 macroporous resin and inexpensive SKP-10-4300 reverse-phase resin first applied for the purification of capsaicin. After simple optimization of loading/elution conditions for D101 macroporous resin chromatography and SKP-10-4300 reverse-phase resin chromatography, the purities of capsaicin were improved from 7 to 85 %. In the two chromatography processes, the recoveries of capsaicin were 93 and 80 % respectively; the productivities of capsaicin were 1.86 and 4.2 (g capsaicin/L resin) per day respectively. It is worth mentioning that a by-product of capsaicin production was also obtained with a high purity (90 %).

• 出版日期2015-1