Adipose Tissue-Derived Stromal Cells Protect Hair Cells From Aminoglycoside

作者：Yoshida Atsuhiro; Kitajiri Shin Ichiro; Nakagawa Takayuki^*; Hashido Kento; Inaoka Takatoshi; Ito Juichi

来源：Laryngoscope, 2011, 121(6): 1281-1286.

DOI：10.1002/lary.21551

• Adipose tissue-derived stromal cell
• protection
• cochlea
• cell therapy

Background: Previous studies have demonstrated the therapeutic paracrine activity of adipose tissue-derived stromal cells (ADSCs). This study aimed to examine the ADSC potential for protecting auditory hair cells from aminoglycoside toxicity via paracrine of multiple growth factors and cytokines.
Study Design: Experimental study.
Methods: We assessed hair cell protection from neomycin toxicity by ADSC-derived factors using an explant culture system, in which cochlear explants and ADSCs were separated by a culture mesh insert to avoid direct contact. We measured the levels of growth factors and cytokines in ADSC culture media using an enzyme-linked immunosorbent assay (ELISA).
Results: Neomycin induced severe degeneration of auditory hair cells in cochlear explants, but co-culture with ADSCs significantly increased the number of surviving hair cells in explants. ELISA analysis revealed that ADSCs secreted insulin-like growth factor-1, nerve growth factor, vascular epithelial growth factor, transforming growth factor beta 1, monocyte chemotactic protein-1, and most prominently hepatocyte growth factor.
Conclusions: These findings demonstrate that ADSCs have the capacity to protect auditory hair cells, and can be a useful strategy to develop therapy for deafness in the clinic. The multiple paracrine growth factors and cytokines secreted by ADSCs might be involved in this effect.

• 出版日期2011-6