A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: performance in vitro and in live cells

作者：Terrones Yamili Toum; Leskow Federico Coluccio; Bordoni Andrea V; Acebedo Sofia L; Spagnuolo Carla C; Wolosiuk Alejandro

来源：Journal of Materials Chemistry B, 2017, 5(22): 4031-4034.

DOI：10.1039/c7tb00622e

We report the synthesis of a near-infrared (NIR) fluorescent pH probe with a remarkable Stokes shift (similar to 135 nm) based on a tricarbocyanine (Cy-PIP). The fluorescent molecule was anchored to SiO2 nanoparticles (Cy-PIP@SiO2) and is capable of monitoring pH changes within the physiological range (pH 6-8). The Cy-PIP@SiO2 nanoparticles were successfully internalized by HeLa cells as shown by fluorescence confocal microscopy, while flow cytometry revealed pH fluctuations during the endocytic pathway.

• 出版日期2017-6-14