Are Lewis b and H Type 1 on Helicobacter pylori Involved in Binding of Bacteria to MUC1 Mucin?

作者：Radziejewska Iwona^*; Borzym Kluczyk Malgorzata; Leszczynska Katarzyna

来源：Advances in Clinical and Experimental Medicine, 2013, 22(3): 347-353.

• Helicobacter pylori
• H type 1
• Lewis b
• MUC1

Background. Helicobacter pylori is responsible for gastroduodenal diseases such as chronic gastritis, gastric and duodenal ulcers and also gastric malignances. During an infection, gastric mucins, especially secretory MUC5AC, are said to participate in interactions with bacterial adhesins. Recently, epithelial MUC1 mucin has also been proposed to be engaged in infection development. H. pylori surface possesses lipopolisaccharides with Lewis antigens whose role in interactions with gastric mucins has not been elucidated thoroughly so far.
Objectives. To check the involvement of Lewis b and H type 1 structures of H. pylori in adhesion of bacteria to MUC1 mucin.
Material and Methods. The study was performed on gastric juices taken from 10 clinical patients and 4 H. pylori strains. Bacteria were assessed for the presence of Lewis b and H type 1 structures by ELISA test. Interactions of H. pylon with MUC1 were analyzed by sandwich ELISA method with bacteria pretreated or not with Lewis b - HSA or H type 1 - HSA glycoconjugates.
Results. In the majority of the patients, the slight increase in adhesion of H. pylon to MUC1, after pretreatment of bacteria with H type 1 glycoconjugate was observed. A statistically significant difference was revealed in one strain with a dose of conjugate 50 mu g/mL (P = 0.05). The influence of Lewis b on adhesion is considered to be contradictory.
Conclusions. H type 1 antigens are suggested to be involved in carbohydrate - carbohydrate interactions of H. pylori with MUC1 mucin

• 出版日期2013-6