A more sensitive platform for the detection of low-abundance BRAF(V600E) mutations

作者：Jiang, Weiqin; Wang, Weibin; Fu, FangFang; Teng, Xiaodong; Wang, Haohao; Wang, Haiyong; Teng, Lisong^*

来源：Molecular and Cellular Biochemistry, 2012, 366(1-2): 49-58.

DOI：10.1007/s11010-012-1282-2

• BRAF
• Allele-specific real-time PCR
• Sanger sequencing
• Pyrosequencing

Identifying low-abundance mutations is important for the therapy and diagnose of cancer. Since the potential for tumor heterogeneity, the efficient detection of cancer-relevant mutations largely depends on the sensitivity of the methods employed. To confirm whether the mutation detection platforms affect the perceived prevalence of the BRAF(V600E) and its correlation with clinicopathologic features in papillary thyroid carcinomas (PTC), we compared Sanger Sequencing (SS), Pyrosequencing (PS), and a newly built allele-specific real-time PCR (AS-qPCR) apparatus for the detection of BRAF(V600E) in a Chinese cohort of conventional variant PTC. Accurate plasmid standards were built to assess the limit of detection of the three platforms. In this research, AS-qPCR has been found both the most sensitive and reliable at detecting mutation. The mutations detected by AS-qPCR which were not detected by SS or PS due to low abundance were confirmed by mutation enrichment platform COLD-PCR followed by SS. When analyzed by AS-qPCR, BRAF(V600E) was associated with a more aggressive phenotype. Our results indicate that the reported prevalence of the BRAF(V600E) mutations in PTC has been underestimated and more sensitive methods such as AS-qPCR should be applied in clinical settings.

• 出版日期2012-7
• 单位浙江大学