Additive protection induced by mixed virus-like particles presenting respiratory syncytial virus fusion or attachment glycoproteins

作者：Lee Sujin; Quan Fu Shi; Kwon Youngman; Sakamoto Kaori; Kang Sang Moo; Compans Richard W; Moore Martin L^*

来源：Antiviral Research, 2014, 111: 129-135.

DOI：10.1016/j.antiviral.2014.09.005

• Respiratory syncytial virus
• Virus like particle (VLP)
• Lung pathology
• RSV fusion (F) protein
• RSV attachment glyco (G) protein

Respiratory syncytial virus (RSV) is the most important pathogen for lower respiratory tract illness in infants and a high priority for vaccine development. We previously reported that RSV virus-like particles (VLPs) expressing either the fusion (F) or attachment (G) glycoprotein could confer protection against RSV challenge in BALB/c mice. Here, we tested the hypothesis that RSV VLP vaccine efficacy can be enhanced by mixing RSV VIP F and RSV VIP G. and we analyzed host responses to these RSV VLPs. Mice were immunized with VLP F, VLP G, or VLP F + VLP G. Lung viral loads in BALB/c mice following RSV strain A2-line19F challenge were-lower in mice vaccinated with RSV VLP F + VIP G compared to VLP F- or VIP G-vaccinated mice. Vaccination with VLP F or VLP F + VLP G induced similar levels of neutralizing antibodies. The enhanced protection against RSV challenge induced by vaccination with RSV VLP F + VIP G correlated with CD8 T cells producing T helper type 1 cytokines. VLP G vaccination alone followed by challenge resulted in immunopathology similar to formalin-inactivated RSV vaccination and RSV challenge. Taken together, mixed VIP F + VIP G provided a high level of protection against RSV without vaccine-induced immunopathology, but VLP G vaccination enhanced disease when used alone.

• 出版日期2014-11