Development and validation of a liquid chromatography-tandem mass spectrometry assay for the simultaneous quantification of buprenorphine, norbuprenorphine, and metabolites in human urine

作者：Kacinko Sherri L; Concheiro Guisan Marta; Shakleya Diaa M; Huestis Marilyn A^*

来源：Analytical and Bioanalytical Chemistry, 2008, 392(5): 903-911.

DOI：10.1007/s00216-008-2326-z

• Buprenorphine
• Norbuprenorphine
• Buprenorphine glucuronide
• Norbuprenorphine glucuronide
• Urine
• Liquid chromatography-tandem mass spectrometry

A liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of buprenorphine (BUP), norbuprenorphine (NBUP), buprenorphine glucuronide (BUP-Gluc), and norbuprenorphine glucuronide (NBUP-Gluc) in human urine was developed and fully validated. Extensive endogenous and exogenous interferences were evaluated and limits of quantification were identified empirically. Analytical ranges were 5-1,000 ng/mL for BUP and BUP-Gluc and 25-1,000 ng/mL for NBUP and NBUP-Gluc. Intra-assay and interassay imprecision were less than 17% and recovery was 93-116%. Analytes were stable at room temperature, at 4 degrees C, and for three freeze-thaw cycles. This accurate and precise assay has sufficient sensitivity and specificity for urine analysis of specimens collected from individuals treated with BUP for opioid dependence.

• 出版日期2008-11