Novel methods to apply micro dynamic stimulations on cultured adhesive cells and its application in constructing gel-embedded three-dimensional neuronal structures differentiated from human iPS cells

作者：Kosawada Tadashi^*; Ohnishi Keita; Satoh Hiroaki; Feng Zhong gang; Goto Kaoru

来源：Microsystem Technologies, 2018, 24(1): 625-638.

DOI：10.1007/s00542-017-3399-4

In cellular engineering, device system to apply active dynamic stimulations on cultured adhesive cells or cell colonies is highly needed in order to control cellular functions as well as morphologies prior to clinical treatment such as cell transplantation in regenerative medicine. In this study, simple but effective cantilever-type three-dimensional micro vibration table as well as two-dimensional one to be used within limited space and environment inside the cell incubator is newly developed to stimulate cultured adhesive iPS cells colony directly to promote their differentiation into objective cells. The finite element method is utilized in developing process. Also, together with the dynamic stimulation, a method of gel-embedded culture for neurons is proposed to construct three-dimensional neuronal structures. Effects of the dynamic stimulations on the differentiation and growth process of the human iPS cells into neuronal cells are investigated by measuring the immunostained areas of beta III-tubulin of the differentiated neural cells with or without the dynamic stimulations. It is found that there is significant difference in differentiation and growth efficiency of the human iPS cells into neurons between the statically cultured cells and the dynamically stimulated ones. Furthermore, it is confirmed that the gel-embedded culture together with the dynamic stimulations is a promising strategy to construct the three-dimensional neuronal structures.

• 出版日期2018-1