Nanometer-scale physically adsorbed thermoresponsive films for cell culture

作者：Healy Deirdre^*; Nash Maria^*; Gorelov Alexander; Thompson Kerry; Dockery Peter; Belochapkine Serguei; Madden Julia; Rochev Yury

来源：International Journal of Polymeric Materials and Polymeric Biomaterials, 2017, 66(5): 221-234.

DOI：10.1080/00914037.2016.1201765

• Cell sheet detachment
• physical adsorption
• poly (N-isopropylacrylamide)
• thermoresponsive polymers

Physical adsorption was used to produce nanometer thick thermoresponsive films with a view to nonenzymatic cell detachment. Two polymers were investigated, poly-(N-isopropylacrylamide) and poly (N-isopropylacrylamide-co-N-tertbutylacrylamide). Substrates were prepared above and below the polymers' LCST to investigate the effect of polymer conformation on the prepared substrates. Endothelial cells were seeded on the prepared films; cell proliferation was higher on the films produced below the polymers' LCST than on those prepared above and cells detached from the surfaces upon temperature reduction. Physical adsorption of poly-(N-isopropylacrylamide)-based films is a viable approach to produce substrates compliant with cell growth and temperature modulated detachment.
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• 出版日期2017