A New Tactic for Label-Free Recognition of beta-Trophin via Electrochemiluminescent Signalling on an AuNPs Supported Immuno-Interface

作者：Zheng, Lijuan; Fang, Chen; Yan, Jilin; Li, Huiling^*; Tu, Yifeng^*

来源：Scientific Reports, 2017, 7(1): 11199.

DOI：10.1038/s41598-017-11750-8

• GOLD NANOPARTICLES
• TITANIA NANOTUBES
• CAPILLARY-ELECTROPHORESIS
• CIRCULATING BETATROPHIN
• ESCHERICHIA-COLI
• CHEMILUMINESCENCE
• LUMINOL
• RADIOIMMUNOASSAY
• IMMUNOSENSORS
• ENHANCEMENT

In this paper, a new strategy is reported for preparing a label-free beta-trophin electrochemiluminescent (ECL) immunosensor with good specificity, reproducibility and stability. An aquagel polymer from the hydrolysis of (3-aminopropyl) trimethoxysilane acted as the linker to catch the Au nanoparticles (AuNPs) on the indium-tin oxide (ITO) substrate by a two-step method. The AuNPs play an important role in enhancing ECL and immobilizing the beta-trophin antibody. This immunosensor can test for beta-trophin using luminol as an ECL probe. The ECL intensity at the resultant sensor, after the direct immuno-interaction, was proportional to the concentration of beta-trophin and had a low limit of quantification as 4.2 ng mL(-1). After deep discussions on the ECL mechanism of this immunosensor, we found that its sensitivity is greatly affected by the presence of oxygen and improved under deoxygenation. We believe that this sensor can be used for clinical cases.

• 出版日期2017-9-11
• 单位苏州大学