Distribution of D-3-aminoisobutyrate-pyruvate aminotransferase in the rat brain

作者：Abe Masao^*; Ochi Shinichiro; Mori Yoko; Yamazaki Kiyohiro; Ishimaru Takashi; Yoshino Yuta; Fukuhara Ryuji; Tanimukai Satoshi; Matsuda Seiji; Ueno Shu ichi

来源：BMC Neuroscience, 2014, 15(1): 53.

DOI：10.1186/1471-2202-15-53

• D-3-aminoisobutyrate-pyruvate aminotransferase
• Asymmetric dimethylarginine
• RT-PCR
• Western blotting
• Immunohistochemistry

Background: D-3-aminoisobutyrate, an intermediary product of thymine, is converted to 2-methyl-3-oxopropanoate using pyruvate as an amino acceptor by D-3-aminoisobutyrate-pyruvate aminotransferase (D-AIB AT; EC 2.6.1.40). A large amount of D-AIB AT is distributed in the kidney and liver; however, small amounts are found in the brain. Recently, D-AIB AT was reported to metabolize asymmetric dimethylarginine (ADMA) in vivo and was suggested to be an important enzyme for nitric oxide metabolism because ADMA is a competitive inhibitor for nitric oxide synthase. In this study, we examined the distribution of D-AIB AT in the rat brain further to understand its role. We measured D-AIB AT mRNA and protein expression using quantitative RT-PCR and Western blotting, and monitored its distribution using immunohistochemical staining. %26lt;br%26gt;Results: D-AIB AT was distributed throughout the brain, with high expression in the cortex and hippocampus. Immunohistochemical staining revealed that D-AIB AT was highly expressed in the retrosplenial cortex and in hippocampal neurons. %26lt;br%26gt;Conclusion: Our results suggest that D-AIB AT is distributed in the examined-just the regions and may play an important role there.

• 出版日期2014-4-27