Lack of endoplasmic reticulum 1,2-alpha-mannosidase activity that trims N-glycan Man(9)GlcNAc(2) to Man(8)GlcNAc(2) isomer B in a manE gene disruptant of Aspergillus oryzae

作者：Akao Takeshi^*; Yahara Akinori; Sakamoto Kazutoshi; Yamada Osamu; Akita Osamu; Yoshida Takashi

来源：Journal of Bioscience and Bioengineering, 2012, 113(4): 438-441.

DOI：10.1016/j.jbiosc.2011.11.015

• Aspergillus oryzae
• N-Glycan processing
• 1,2-alpha-Mannosidase
• Endoplasmic reticulum
• Glycoprotein

The gene manE, encoding a probable class I endoplasmic reticulum 1,2-alpha-mannosidases (ER-Man), was identified from the filamentous fungus Aspergillus oryzae due to similarity to orthologs. It removes a single mannose residue from Man(9)GlcNAc(2), generating Man(8)GlcNAc(2) isomer B. Disruption of manE caused drastic decreases in ER-Man activity in A. oryzae microsomes.

• 出版日期2012-4