Elucidation of the Molecular-Mechanisms and In Vivo Evaluation of the Anti-inflammatory Effect of Alginate-Derived Seleno-polymannuronate

作者：Bi, Decheng; Lai, Qiuxian; Cai, Nan; Li, Tong; Zhang, Yiyao; Han, Qingguo; Peng, Yanwen; Xu, Hong; Lu, Jun; Bao, Weiyang; Liu, Qiong; Xu, Xu^*

来源：Journal of Agricultural and Food Chemistry, 2018, 66(9): 2083-2091.

DOI：10.1021/acs.jafc.7b05719

• seleno-polymannuronate
• lipopolysaccharide
• anti-inflammation
• nuclear factor kappa B
• mitogen-activated protein kinase

Alginate-derived polymannuronate (PM) is a type of polysaccharide found in edible brown seaweeds. Seleno-polymannuronate (Se-PM) was prepared from PM via synthesis using sulfation- and selenation-replacement reactions. The anti-inflammatory activity of Se-PM and its corresponding molecular mechanisms were investigated. In lipopolysaccharide (LPS)-activated murine macrophage RAW264.7 cells, Se-PM significantly attenuated the production of nitric oxide (NO), prostaglandin E-2 (PGE(2)), and reactive oxygen species (ROS); the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2); and the secretion of proinflammatory cytokines. Moreover, Se-PM remarkably suppressed the LPS-induced activation of the nuclear-factor (NF)-kappa B and mitogen-activated-protein-kinase (MAPK) signaling pathways in RAW264.7 cells. Furthermore, Se-PM also decreased the production of proinflammatory mediators in LPS-triggered primary murine macrophages. Additionally, Se-PM inhibited the inflammatory response in the air-pouch inflammation model. These results might contribute to the overall understanding of the potential health benefits of Se-PM for food and drug applications.

• 出版日期2018-3-7
• 单位深圳大学; 中山大学