Study of optical anisotropy in thin molecular layers by total internal reflection ellipsometry

作者：Balevicius Z^*; Makaraviciute A; Babonas G J; Tumenas S; Bukauskas V; Ramanaviciene A; Ramanavicius A

来源：Sensors and Actuators B: Chemical , 2013, 181: 119-124.

DOI：10.1016/j.snb.2013.01.059

• Total internal reflection ellipsometry
• Spectroscopic ellipsometry
• Surface plasmons
• Antibody-antigen

Variable angle total internal reflection ellipsometry (TIRE) has been utilized to reveal anisotropy in the optical response of fragmented antibody (frag-Ab) monolayer. Optical anisotropy has been taken into account in determination of optical constants of an organic layer. The orientation of frag-Ab on the surface has been characterized by using the uniaxial model in the analysis of TIRE data. Regression results have shown that the largest dimension of frag-Ab molecule (11.5 nm) is oriented parallel to the gold surface with a slight tilt (13 degrees). Due to the tilt, the distance between the recognition sites of frag-Ab and the gold surface increases resulting in an enhanced reactivity of the molecules and better steric accessibility.

• 出版日期2013-5