Avanti lipid tools: Connecting lipids, technology, and cell biology

作者：Sims Kacee H^*; Tytler Ewan M; Tipton John; Hill Kasey L; Burgess Stephen W; Shaw Walter A

来源：Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids, 2014, 1841(8): 1038-1048.

DOI：10.1016/j.bbalip.2014.05.008

• Lipid toolbox
• Protein-lipid interactions
• Lipid-binding antibodies
• Gold-conjugated lipids
• Lipidomics
• Mass spectrometry

Lipid research is challenging owing to the complexity and diversity of the lipidome. Here we review a set of experimental tools developed for the seasoned lipid researcher, as well as, those who are new to the field of lipid research. Novel tools for probing protein-lipid interactions, applications for lipid binding antibodies, enhanced systems for the cellular delivery of lipids, improved visualization of lipid membranes using gold-labeled lipids, and advances in mass spectrometric analysis techniques will be discussed. Because lipid mediators are known to participate in a host of signal transduction and trafficking pathways within the cell, a comprehensive lipid toolbox that aids the science of lipidomics research is essential to better understand the molecular mechanisms of interactions between cellular components. This article is part of a Special Issue entitled Tools to study lipid functions.

• 出版日期2014-8