Anti-oxidative and Neuroprotective Activities of Pig Skin Gelatin Hydrolysates

作者：Kim Dong Wook; Park Kimoon; Ha Goeun; Jung Ju Ri; Chang Ounki; Ham Jun Sang; Jeong Seok Geun; Park Beom Young; Song Jin; Jang Aera^*

来源：Korean Journal for Food Science of Animal Resources, 2013, 33(2): 258-267.

DOI：10.5851/kosfa.2013.33.2.258

• gelatin hydrolysates
• collagen
• neuroblastoma cell
• acetylcholine esterase
• pig skin

This study was conducted to determine the antioxidative and neuroprotective effect of pig skin extracts (PS) and pig skin gelatin hydrolysates (LPS) using a human neuroblastoma cell line (SH-SY5Y). The extraction yield of PS was 3 fold higher than that of LPS. The protein content of PS was about 10 fold higher than that of LPS (p %26lt; 0.05). Also LPS increased antioxidative activity dose dependently, and the activity was significantly higher than PS at all concentration (p %26lt; 0.05). DPPH radical scavenging activity of LPS at 50 mg/mL was 92.97%, which was similar to 1 mu M vitamin C as a positive control. ABTS radical scavenging activity of LPS (20 mg/mL) was 89.83% and oxygen radical absorbance capacity of LPS at 1 mg/ mL was 141.39 mu M Trolox Equvalent/g. No significant change of human neuroblastoma cells was determined by MTT test. Cell death by oxidative stress induced by H2O2 and amyloid beta 1-42 (A beta(1-42)) was protected by LPS rather than PS. Acetylcholine esterase was significantly inhibited, by up to 33.62% by LPS at 10 mg/mL. Therefore, these results suggest that pig skin gelatin hydrolysates below 3 kDa have potential to be used as anti-oxidative and neuroprotective functional additives in the food industry, while further animal test should be determined in the future.

• 出版日期2013-4