Anti-tumor activity of curcumin against androgen-independent prostate cancer cells via inhibition of NF-kappa B and AP-1 pathway in vitro

作者：Liu, Shuanglin; Wang, Zhihua; Hu, Zhiquan^*; Zeng, Xing; Li, Youyuan; Su, Yaowu; Zhang, Chuanhua; Ye, Zhangqun

来源：Journal of Huazhong University of Science and Technology-Medical Sciences, 2011, 31(4): 530-534.

DOI：10.1007/s11596-011-0485-1

• curcumin
• androgen-independent prostate cancer
• NF-kappa B
• AP-1

The anti-tumor activity of curcumin against androgen-independent prostate cancer cells in vitro and the possible mechanism were investigated. After curcumin treatment, the effect of curcumin on the proliferation of prostate cancer PC-3 cells was assessed by CFSE staining. Flow cytometery (FCM) was performed to analyze the cell cycle and the induction of apoptosis of tumor cells. A luciferase reporter gene assay was used to determine the effects of curcumin on the activities of intracellular NF-kappa B and AP-1 signaling pathways. The results showed curcumin could effectively inhibit the proliferation of PC-3 cells in vitro (P < 0.05). Cells were arrested at G(2)/M phase. After curcumin treatment, the percentage of apoptotic cells was significantly higher than in control group (P < 0.05). The results of the luciferase assay revealed that curcumin selectively inhibited the activities of the NF-kappa B and AP-1 signaling pathways in PC-3 cells significantly. It was suggested that curcumin could exert anti-tumor activity against androgen-independent prostate cancer cells in vitro by inhibiting cellular proliferation and inducing apoptosis, which was probably contributed to the inhibition of transcription factors NF-kappa B and AP-1.

• 出版日期2011-8
• 单位武汉市中西医结合医院; 华中科技大学