Analysis of urinary metabolites for breast cancer patients receiving chemotherapy by CE-MS coupled with on-line concentration

作者：Yu, Lanfang; Jiang, Cheng; Huang, Sui; Gong, Xingchu; Wang, Shufang; Shen, Peng^*

来源：Clinical Biochemistry, 2013, 46(12): 1065-1073.

DOI：10.1016/j.clinbiochem.2013.05.049

• CE-MS
• Organic acid
• Amino acid
• Urine
• Breast cancer

Objective: This study aimed to explore the relationship between urinary metabolites and clinical chemotherapy response in breast cancer by CE-MS coupled with on-line concentration. @@@ Design and methods: Urine samples were obtained from patients with advanced or locally advanced breast cancer (n = 21) before and after chemotherapy and healthy volunteers (n = 21). A rapid and sensitive hexadimethrine bromide-coating CE-MS method coupled with normal stacking is developed for the determination of organic acids in human urine. Another CE-MS method coupled with pH-mediated sample stacking is used for the analysis of amino acids and organic acids. @@@ Results: After receiving chemotherapy, chemotherapy-sensitive patients showed 30% change in metabolite levels compared to healthy people, while chemotherapy-insensitive patients showed only 9% change in metabolite levels compared to healthy people showing recurrence. The extent of energy insufficiency for chemotherapy-insensitive patients was greater than that for chemotherapy-sensitive patients. @@@ Conclusions: Urinary metabolic products may be new potential predictive markers for therapy efficacy. However, more studies with a larger sample size are required to confirm these conclusions.

• 出版日期2013-8
• 单位浙江大学