An efficient method for extraction, separation and purification of eugenol from Eugenia caryophyllata by supercritical fluid extraction and high-speed counter-current chromatography

作者：Geng Yanling; Liu Jianhua; Lv Ruimin; Yuan Jinpeng; Lin Yunliang; Wang Xiao^*

来源：Separation and Purification Technology, 2007, 57(2): 237-241.

DOI：10.1016/j.seppur.2007.04.015

• supercritical fluid extraction (SFE)
• counter-current chromatography
• Eugenia caryophyllata
• eugenol
• preparative chromatography

Supercritical fluid extraction (SFE) of eugenol from Eugenia caryophyllata was performed. The optimization of parameters was carried out using an analytical-scale supercritical fluid extraction system. Then the extraction was scaled up by 60x using a preparative SFE system under the optimized conditions of 50 degrees C, 30 MPa and a sample particle size of 40-60 mesh. The yield of the preparative SFE was 17.1 % and the yield of eugenol was 94 mg/g of dry buds. Eugenol in the extract was separated and purified by high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:0.5:1:0.5, v/v). From 1.5 g of crude extract, 804 mg of eugenol was obtained at 98.5% purity as determined by HPLC.

• 出版日期2007-10-15
• 单位山东省科学院