A nucleic acid biosensor for the detection of a short sequence related to the hepatitis B virus using bis(benzimidazole)cadmium(II) dinitrate as an electrochemical indicator

作者：Li Xue Mei; Ju Heng Qiang; Du Li Ping; Zhang Shu Sheng^*

来源：Journal of Inorganic Biochemistry, 2007, 101(8): 1165-1171.

DOI：10.1016/j.jinorgbio.2007.05.003

• hepatitis B virus
• electrochemical DNA biosensor
• Bis(benzimidazole)cadmium(II) dinitrate

A novel hybridization indicator, bis(benzimidazole)cadmium(II) dinitrate (Cd(bzim)2(NO3)(2)), was utilized to develop an electrochemical DNA biosensor for the detection of a short DNA sequence related to the hepatitis B virus (HBV). The sensor relies on the immobilization and hybridization of the 21-mer single-stranded oligonucleotide from the HBV long repeat at the glassy carbon electrode (GCE). The hybridization between the probe and its complementary sequence as the target was studied by enhancement of the peak of the Cd(bziM)(2+)(2) indicator using cyclic voltarnmetry (CV) and differential pulse voltarnmetry (DPV). Numerous factors affecting the probe immobilization, target hybridization, and indicator binding reactions were optimized to maximize the sensitivity and speed of the assay time. With this approach, a sequence of the HBV could be quantified over the range from 1.49 x 10(-7) M to 1.06 x 10(-6) M, with a linear correlation of r = 0.9973 and a detection limit of 8.4 x 10(-8) M. The Cd(bzim)(2+)(2) signal observed from the probe sequence before and after hybridization with a four-base mismatch containing sequence was lower than that observed after hybridization with a complementary sequence, showing good selectivity. These results demonstrate that the Cd(bzim)(2+)(2) indicator provides great promise for the rapid and specific measurement of the target DNA.

• 出版日期2007-8
• 单位青岛科技大学