科研之友
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摘要
A visualized assay for quercetin (QU) was first developed based on the formation of silver-gold alloy nanoparticles in this contribution. With the ability to reduce metal ions to metal substances, QU could reduce Ag+ absorbed on the surface of gold nanoparticles to metallic silver. The thickness of the formed Ag shell and the color change of the solution were proportional to the concentration of QU. Therefore, visualized detection of QU could be realized by studying the surface resonance plasmon absorption spectra of the analytical systems after addition of different concentration of QU. Under optimum conditions, trace amount of QU could be detected in the linear range 9.0x10(-7)-1.0x10(-4) mol L-1 with a detection limit of 6.5x10(-7) mol L-1. The present assay was applied in the determination of QU in human serum and satisfactory results were obtained. This assay is simple, rapid, and cost-effective, and it is a powerful complement for the spectroscopy assays for QU. Also, it is the first visualized spectroscopic assay of QU until now.
