A chemical platform for improved induction of human iPSCs

作者：Lin Tongxiang; Ambasudhan Rajesh; Yuan Xu; Li Wenlin; Hilcove Simon; Abujarour Ramzey; Lin Xiangyi; Hahm Heung Sik; Hao Ergeng; Hayek Alberto; Ding Sheng^*

来源：Nature Methods, 2009, 6(11): 805-U24.

DOI：10.1038/NMETH.1393

The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (>200-fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, nonviral methods for reprogramming human somatic cells.

• 出版日期2009-11