Mulberry (Morus atropurpurea Roxb.) leaf polyphenols inhibits adipogenesis and lipogenesis-related gene expression in 3T3-L1 adipocytes

作者：Li, Qian; Dai, Yanli; Zou, Yuxiao^*; Liao, Sentai^*; Shen, Weizhi; Hu, Tenggen; Liu, Fan

来源：Journal of Food Biochemistry, 2018, 42(5): e12599.

DOI：10.1111/jfbc.12599

• 3T3-L1 adipocytes
• antiobesity
• lipid accumulation
• Morus atropurpurea Roxb
• Mulberry leaf polyphenols

The effects of mulberry leaf polyphenols (MLPs) on the proliferation and differentiation of 3T3-L1 preadipocytes were investigated in this study. No significant inhibitory effect on cell proliferation when MLP content was 10-50 g/mL. MLP inhibited lipid accumulation in 3T3-L1 preadipocytes dose-dependently at concentrations of 0-50 g/mL. The treatment of differentiating 3T3-L1 cells with MLP at concentration of 40-50 g/mL significantly inhibited the spillage of free fatty acids (FFAs), reduced the intracellular total triglyceride (TG) and cholesterol (TC) concentration. Furthermore, a decrease in mRNA expression of peroxisome proliferator activated receptor- and CCAAT-enhancer-binding protein-, as well as fatty acid synthase and adiponectin were observed. This is the first study to demonstrate that MLP attenuated adipogenesis-related mRNA expression of 3T3-L1 preadipocytes. The findings suggested that MLP has complimentary potency for the regulation of obesity.
Practical application
The effects of MLP on the proliferation and differentiation of 3T3-L1 preadipocytes were investigated for the first time in this study. MLP decreased FAA, TC, TG, lipid accumulation and attenuated adipogenesis-related mRNA expression in differentiating 3T3-L1 cells. The result clearly identified mulberry leaf supplements could be used as a nutraceutical agent to ameliorate obesity and its complications

• 出版日期2018-10
• 单位广东省农业科学院