科研之友
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摘要
Fibroblast growth factor (FGF) 8b interacts with its receptors and promotes angiogenesis in hormone-dependent tumors. In the present study, we demonstrated that a short peptide, termed 8b-13, which mimics part of the FGF8b structure, significantly inhibited the proliferation and migration of human umbilical vein endothelial cells (HUVECs) triggered by FGF8b using 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazo-lium bromide (MTT), flow cytometry and an in vitro scratch assay. In addition, the findings from western blotting and reverse transcription-quantitative polymerase chain reaction revealed that 8b-13 appeared to counteract the effects of FGF8b on the expression of cyclin D1, the activation of signaling cascades, and the expression of proangiogenic factors; these actions may be involved in the mechanism underlying the inhibitory effects of 8b-13 on FGF8b-induced HUVEC proliferation and migration. The present results suggested that 8b-13 may be considered a potent FGF8b antagonist with antiangiogenic activity, and may have potential as a novel therapeutic agent for the treatment of cancer characterized by abnormal FGF8b upregulation.
