A new process of IgG purification by negative chromatography: Adsorption aspects of human serum proteins onto omega-aminodecyl-agarose

作者：Lazzarotto Bresolin Igor Tadeu; Martins de Souza Maria Cristiane; Alves Bueno Sonia Maria

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2010, 878(23): 2087-2093.

DOI：10.1016/j.jchromb.2010.06.009

• Adsorption
• Purification
• Human IgG
• Mixed-mode interactions
• omega-Aminodecyl-agarose

The adsorbent omega-aminodecyl-agarose was evaluated as to its feasibility for the adsorption of human serum and plasma proteins, aiming at the purification of immunoglobulin G (IgG). The contribution of electrostatic and hydrophobic interactions (mixed-mode) and the effects of buffer system on the adsorption of serum proteins were also studied. The adsorption isotherm parameters of human serum albumin (HSA) and IgG were evaluated, pointing to the existence of cooperative effects in the process. A positive (n = 2.30 +/- 0.38) and negative cooperativity (n = 0.63 +/- 0.12) were observed for IgG and HSA binding, respectively. High purity IgG was obtained (based on total protein concentration and nephelometric analysis of HSA transferrin, and immunoglobulins A. G, and M) with a 75% recovery in Hepes 25 mmol L(-1) pH 6.8 feeding human serum. These results indicate that the use of omega-aminodecyl-agarose is a potential technique for purification of IgG from human serum.

• 出版日期2010-8-1