Automated identification and quantification of glycerophospholipid molecular species by multiple precursor ion scanning

作者：Ejsing Christer S; Duchoslav Eva; Sampaio Julio; Simons Kai; Bonner Ron; Thiele Christoph; Ekroos Kim; Shevchenko Andrej^*

来源：Analytical Chemistry, 2006, 78(17): 6202-6214.

DOI：10.1021/ac060545x

We report a method for the identification and quantification of glycerophospholipid molecular species that is based on the simultaneous automated acquisition and processing of 41 precursor ion spectra, specific for acyl anions of common fatty acids moieties and several lipid class-specific fragment ions. Absolute quantification of identified species was linear within a concentration range of 10 nM-100 mu M and was achieved by spiking into total lipid extracts a set of synthetic lipid standards with diheptadecanoyl (17:0/17: 0) fatty acid moieties, representing six common classes of glycerophospholipids. The automated analysis of total lipid extracts was powered by a robotic nanoflow ion source and produced currently the most detailed description of the glycerophospholipidome.

• 出版日期2006-9-1