A standardized immunofluorescence test method with human neutrophil antigen-expressing cell lines to enhance antibody detection

作者：Lopez G H^*; Dean M M; Yasui K; Schuller R M; Hirayama F; Fung Y L

来源：Vox Sanguinis, 2012, 102(2): 171-174.

DOI：10.1111/j.1423-0410.2011.01532.x

• cell lines
• immunofluorescence
• neutrophil antibody screening

There is an international need for a large-scale human neutrophils antigen (HNA) antibody screening platform to minimize the risk of antibody-mediated transfusion-related acute lung injury. However, sourcing a substantial, reliable source of HNA, as well as the scarcity of well-characterized HNA antisera for validating new screening platforms, remain as major obstacles. This short communication presents an improved protocol for the effective use of HNA-expressing KY cells as a screening platform using eight well-characterized HNA antisera of a single defined specificity.

• 出版日期2012-2