Postprandial whole-body glycolysis is similar in insulin-resistant and insulin-sensitive non-diabetic humans

作者：Galgani J E^*; Ravussin E

来源：Diabetologia, 2012, 55(3): 737-742.

DOI：10.1007/s00125-011-2413-0

• Glucose metabolism
• Glucose tolerance
• Glycolysis
• Insulin resistance
• Insulin secretion

Insulin resistance is characterised by impaired glucose utilisation when measured by a euglycaemic-hyperinsulinaemic clamp. We hypothesised that, in response to postprandial conditions, non-diabetic individuals would have similar intracellular glycolytic and oxidative glucose metabolism independent of the degree of insulin resistance. %26lt;br%26gt;Fourteen (seven male) sedentary, insulin-sensitive participants (mean +/- SD: BMI 25 +/- 4 kg/m(2); age 39 +/- 10 years; glucose disposal rate 9.4 +/- 2.1 mg [kg estimated metabolic body size](-1) min(-1)) and 14 (six male) sedentary, non-diabetic, insulin-resistant volunteers (29 +/- 4 kg/m(2); 34 +/- 13 years; 5.3 +/- 1.2 mg [kg estimated metabolic body size](-1) min(-1)) received after a 10 h fast 60 g glucose plus 15 g [6,6-H-2(2)]glucose. Serum glucose and insulin concentrations, plasma H-2 enrichment and whole-body gas exchange were determined before glucose ingestion and hourly thereafter for 4 h. Plasma (H2O)-H-2 production is an index of glycolytic disposal. On day 2, participants received a weight-maintenance diet. On day 3, a euglycaemic-hyperinsulinaemic clamp was performed. %26lt;br%26gt;Insulin-resistant individuals had about a twofold higher postprandial insulin response than insulin-sensitive individuals (p = 0.003). Resting metabolic rate was similar in the two groups before (p = 0.29) and after (p = 0.33-0.99 over time) glucose ingestion, whereas a trend for blunted glucose-induced thermogenesis was observed in insulin-resistant vs insulin-sensitive individuals (p = 0.06). However, over the 4 h after the 75 g glucose ingestion, glycolytic glucose disposal was the same in insulin-sensitive and insulin-resistant individuals (36.5 +/- 3.7 and 36.2 +/- 6.4 mmol, respectively; p = 0.99). Similarly, whole-body carbohydrate oxidation did not differ between the groups either before or after glucose ingestion (p = 0.41). %26lt;br%26gt;Postprandial hyperinsulinaemia and modest hyperglycaemia overcome insulin resistance by enhancing tissue glucose uptake and intracellular glucose utilisation.

• 出版日期2012-3